Buy Acridine Orange HCL (65-61-2) MF: C17H19N3 · HCl | MW: 301.81. Get High Quality Acridine Orange HCL (65-61-2) from TriStains.
Acridine Orange HCl is a salt form of Acridine Orange, a fluorescent dye commonly used in biological and biomedical research. It is derived from the acridine family of dyes and shares similar applications and properties with Acridine Orange Base.
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Applications: Fluorescent Staining: Acridine Orange HCl is widely used as a fluorescent dye for staining nucleic acids, specifically DNA and RNA, in cells and tissues. It can intercalate into the DNA or RNA molecules and emit fluorescence when excited with the appropriate wavelengths of light. This allows for the visualization and analysis of nucleic acids under a fluorescence microscope.Cell Viability and Cytotoxicity Assays: Similar to Acridine Orange Base, Acridine Orange HCl is used to assess cell viability and cytotoxicity. It can differentiate between live cells (green fluorescence) and apoptotic or dead cells (orange-red fluorescence) based on their staining patterns. This enables researchers to evaluate cell health, viability, and the effects of various treatments or conditions.Nucleic Acid Quantification: Acridine Orange HCl can be employed for quantifying nucleic acids in solution. By binding to DNA or RNA, it emits fluorescence that can be measured to determine the concentration of nucleic acids present. This is useful in applications such as nucleic acid extraction, purification, and quantification.DNA/RNA Visualization and Analysis: Acridine Orange HCl is used for visualizing and analyzing DNA and RNA in various experimental techniques. It can be utilized in gel electrophoresis, fluorescence in situ hybridization (FISH), and other assays to detect and analyze nucleic acids. Its fluorescence properties enable the identification and examination of DNA or RNA size, quantity, and distribution.Microscopy and Imaging: Acridine Orange HCl is used in fluorescence microscopy and imaging techniques to visualize and study cellular structures, including nuclei, chromosomes, and organelles. It labels nucleic acids within the cell, aiding in the visualization of various cellular components.
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