Buy Amido Black 10B (1064-48-8) MF: C22H14N6O9S2•2Na | MW: 616.49. Get High Quality Amido Black 10B (1064-48-8) from TriStains.
Amido Black 10B is a commonly used synthetic dye that has a deep blue to black color. It is also known by other names such as Acid Black 1, C.I. 20470, and Acid Black 10B. The “10B” in its name refers to its identification number in the Color Index (CI) system.Amido Black 10B is primarily used as a histological and protein stain in various scientific and forensic applications.
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Applications: Staining Properties: Amido Black 10B has an affinity for proteins, particularly albumin and globulin. It binds to the proteins, forming a stable complex that results in a visible stain.Protein Staining: In protein gel electrophoresis techniques like SDS-PAGE, Amido Black 10B is used to visualize protein bands. After the electrophoresis process, the gel is stained with Amido Black 10B, which binds to the proteins present in the gel, allowing researchers to visualize and analyze the protein bands.Sensitivity: Amido Black 10B is known for its high sensitivity, allowing the detection of even small amounts of protein. This property makes it useful in applications where low protein concentrations need to be detected.Bloodstain Detection: In forensic science, Amido Black 10B is employed to enhance the visibility of bloodstains. It reacts with the heme component of blood, resulting in a dark blue or black color that makes it easier to locate and document bloodstains at crime scenes.Limitations: While Amido Black 10B is a widely used staining agent, it has some limitations. It may not stain all types of proteins equally, and the intensity of staining can vary depending on the protein composition. Additionally, it may interfere with downstream applications such as mass spectrometry analysis.
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